Classical and Basal Cell Culture Media

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Gibco™ RPMI 1640 Medium

RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer.

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Cell Line	HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas
Form	Liquid
Without Additives	No HEPES,No Sodium Pyruvate
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Concentration	1 X
Sterility	Sterile-filtered
Cell Type	Leukemic Cells
Sterilization Method	Sterile-filtered
With Additives	Glutamine,Phenol Red
Product Type	RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
Classification	Animal Origin-free
Shelf Life	12 Months From Date of Manufacture
Product Line	Gibco
Serum Level	Standard Serum Supplementation

Gibco™ Williams' E Medium, no phenol red

William's E Medium was originally developed by Williams and Gunn as reduced serum-supplemented medium for long-term cell cultures of adult rat liver epithelial cells. Gibco™ William's E Medium can also be used for growing hepatocyte cells of different species, such as human derived HepaRG™ cells.

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Gibco™ MEM α, nucleosides

MEM α (Minimum Essential Medium α) is widely used for mammalian cell culture as well as selection for transfected DHFR-negative cells.

Pricing & Availability
Specifications

Sterilization Method	Sterile-filtered
Product Type	MEM α (Minimum Essential Medium α)
Form	Liquid
Without Additives	No HEPES
Classification	Animal Origin-free
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Concentration	1 X
Sterility	Sterile-filtered
Product Line	Gibco
Serum Level	Standard Serum Supplementation

Gibco™ AIM V™ Medium (research grade), AlbuMAX™ Supplement

AIM V™ Medium (research grade), AlbuMAX™ Supplement

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Gibco™ FreeStyle™ F17 Expression Medium

An animal origin-free, chemically defined, protein-free medium specifically developed to support the growth and transfection of 293-F and CHO cells in suspension without adaptation.

Pricing & Availability
Specifications

Cell Line	HEK293
Culture Environment	CO₂
Purity or Quality Grade	Qualified for Further Manufacturing
Form	Liquid
Without Additives	No Glutamine
Endotoxin Level	< 1 EU/mL
Concentration	1 X
For Use With (Application)	Protein expression, viral vector production
Sterility	Sterile
Product Type	Expression Medium
Classification	Animal Origin-free,Chemically-defined,Protein-free,Serum-free
Culture Type	Suspension Cell Culture
Product Line	Freestyle
Serum Level	Serum-free

Gibco™ DMEM, powder, high glucose, pyruvate

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Cell Line	HeLa, 293, Cos-7, and PC-12
Content And Storage	Storage conditions: 2-8° C Shipping conditions: Ambient Shelf life: 36 months from date of manufacture
Form	Powder
Without Additives	No HEPES,No Sodium Bicarbonate
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Sterility	Sterile-filtered
Cell Type	Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
With Additives	High Glucose,Glutamine,Phenol Red,Sodium Pyruvate
Product Type	DMEM (Dulbecco's Modified Eagle Medium)
Classification	Animal Origin-free
Shelf Life	36 Months From Date of Manufacture
Product Line	Gibco
Serum Level	Standard Serum Supplementation

Gibco™ Advanced DMEM/F-12

Advanced DMEM/F-12 (Dulbecco's Modified Eagle Medium/Ham's F-12) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation.

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Cell Line	MRC-5, SP2, Vero, WI-38 and Jurkat
Content And Storage	Storage conditions: 2-8° C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture
Form	Liquid
Without Additives	No Glutamine,No HEPES
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Concentration	1 X
Sterility	Sterile-filtered
Sterilization Method	Sterile-filtered
With Additives	High Glucose,Phenol Red,Sodium Pyruvate,Non Essential Amino Acids (NEAA)
Product Type	DMEM (Dulbecco's Modified Eagle Medium)/F-12
Classification	Animal Origin
Shelf Life	12 Months From Date of Manufacture
Product Line	Gibco
Serum Level	Reduced Serum

Corning™ Improved MEM (Richter's Mod.), 1X

With L-Glutamine

Pricing & Availability
Specifications

Content And Storage	2° to 8°C
pH	7 to 7.4
Quantity	6 x 500 mL

Gibco™ Leibovitz's L-15 Medium, GlutaMAX™ Supplement

Leibovitz's L-15 Medium supports the growth of HEP-2 monkey kidney cells and primary explants of embryonic and adult human tissue. L-15 is buffered by phosphates and free base amino acids instead of sodium bicarbonate.

Pricing & Availability
Specifications

Product Type	Leibovitz's L-15 Medium
Form	Liquid
Without Additives	No HEPES,No Sodium Bicarbonate,No Glucose
pH	7.4 to 7.9
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Shelf Life	12 Months
Concentration	1 X

Gibco™ DMEM, low glucose, pyruvate, no glutamine, no phenol red

Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells.

Pricing & Availability
Specifications

Cell Line	HeLa, 293, Cos-7, and PC-12
Form	Liquid
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Concentration	1 X
Sterility	Sterile-filtered
Product Line	Gibco
Serum Level	Standard Serum Supplementation
Cell Type	Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells

Gibco™ AIM V™ Medium, liquid (research grade)

AIM V (research grade) is a medium designed for the proliferation and/or manipulation of T cells, dendritic cells, and other immune cells.

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Content And Storage	Store in refrigerator (2–8°C) and protect from light.
Purity or Quality Grade	Research Grade
Included Antibiotics	Streptomycin Sulfate, Gentamicin
With Additives	Glutamine,Phenol Red,HSA
Product Type	Serum Free Medium (SFM)
Form	Liquid
Culture Type	Mammalian Cell Culture
Product Line	AIM V
Cell Type	Monocytes, Dendritic Cells, T Cells, Hybridomas, PBMCs, Fibroblasts, Macrophages, Myeloma Cells, Lymphocytes

Gibco™ DMEM, high glucose, no glutamine, no calcium

Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells.

Pricing & Availability

Gibco™ DMEM/F-12, HEPES

Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

Pricing & Availability
Specifications

Sterilization Method	Sterile-filtered
With Additives	High Glucose,Glutamine,HEPES,Phenol Red,Sodium Pyruvate
Product Type	DMEM (Dulbecco's Modified Eagle Medium)/F-12
Form	Liquid
Classification	Animal Origin-free
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Concentration	1 X
Sterility	Sterile-filtered

Promotions Available

Gibco™ MEM α, nucleosides, no ascorbic acid

MEM α (Minimum Essential Medium α) is widely used for mammalian cell culture as well as selection for transfected DHFR-negative cells.

Pricing & Availability

Gibco™ RPMI 1640 Medium, powder

RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer.

Pricing & Availability

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Classical and Basal Cell Culture Media

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Gibco™ RPMI 1640 Medium Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ Williams' E Medium, no phenol red Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ MEM α, nucleosides Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ AIM V™ Medium (research grade), AlbuMAX™ Supplement Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ FreeStyle™ F17 Expression Medium Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ Advanced DMEM/F-12 Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ Leibovitz's L-15 Medium, GlutaMAX™ Supplement Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ DMEM, low glucose, pyruvate, no glutamine, no phenol red Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ AIM V™ Medium, liquid (research grade) Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ DMEM, high glucose, no glutamine, no calcium Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ DMEM/F-12, HEPES Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ MEM α, nucleosides, no ascorbic acid Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More